Small amounts of nucleic acids and few cells are required for efficient transformation
No carrier DNA is needed and no extraneous genes or proteins are delivered
Codelivery of multiple plasmids and high levels of cotransformation are possible
Large DNA fragments may be transferred as well as small interfering RNA's for gene silencing
Many cells types can be transfected, including non-dividing cells and plants
Potentially toxic treatments such as using viruses or chemical are avoided
DISADVANTAGES
Cause cell damage, for example when pulse are of wrong length or intensity it may cause pores of cell becomes too large or causing cell damage or rupture
Non-specific transport, in which transport of material "in" and "out" of cell during the time of electropermeability is relatively nonspecific. This may result in ion imbalance that could lead to improper cell function and cell death.
Frequency of transformation is low
Integration is low
Requirement of equipment and skill personal is require